How to use the report?
The report contains several parts, please see the following plot to get more detail about the report
Summary
Unique Genes: 50702
Unique Isoforms: 149434
Splice Junction Classification
Gene Characterization
Transcript Length
Normalized Classification
Splice Junction Characterization
Distribution
Distribution of Splice Junctions by Structural Classification
Distribution of Transcripts by Splice Junctions
RT−Switching
RT−Switching All Junctions
Unique Junctions RT−switching
Comparison With Annotated TSS and TTS
Distance to Annotated Polyadenylation Site
Distance to Annotated Transcription Start Site
Comparison With Annotated TSS and TTS by Subcategories
Distance to Annotated Polyadenylation Site
Alternative 3’End for FSM
Alternative 3’5’End for FSM
Alternative 5’End for FSM
Alternative 3’End for ISM
Alternative 3’5’End for ISM
Alternative 5’End for ISM
Distance to Annotated Transcription Start Site
Alternative 3’End for FSM
Alternative 3’5’End for FSM
Alternative 5’End for FSM
Alternative 3’End for ISM
Alternative 3’5’End for ISM
Alternative 5’End for ISM
PolyA Distance Analysis
PolyA Distance Analysis by categories
PolyA Distance Analysis by subcategories
CAGE distances analysis
Multi-exonic FSM
Distance to CAGE peak of multi-exonic FSM
Multi-exonic ISM
Distance to CAGE peak of multi-exonic ISM
Multi-exonic NIC/NNC
Distance to CAGE peak of multi-exonic NIC
Distance to CAGE peak of multi-exonic NNC
Redundancy Analysis
Only FSM
FSM with a polyA motif found
FSM with CAGE support and polyA motif
Only ISM
ISM with a polyA motif found
ISM with CAGE support and polyA motif
FSM and ISM
FSM and ISM with CAGE support
FSM and ISM with a polyA motif found
FSM and ISM with CAGE support and polyA motif
Intra-Priming Quality Check
Features of Bad Quality
Structural Categories
Quality Control Attributes Across Structural Categories
Features of Good Quality
Attributes
Good Quality Control Attributes